# AOD-9604 Dosage Research: Doses Studied by Species and Route

> AOD-9604 dosage in research context only: the doses, routes, and durations used in mouse, rabbit, and human studies, plus the ~3-minute half-life. No human dosing guidance.

Study-attributed numbers only. No human dosing guidance — the literature gives none.

## Before the details

This page reports AOD-9604 dosage only the way studies report it: a number, a species, and a route. It is not a how-to. There is no approved human dose, because the molecule is not approved, and the one human program that set doses did not produce a weight-loss benefit.

In plain terms: scientists gave AOD-9604 to mice, rabbits, and (in tablet form) to obese adults at a range of amounts. The human trials covered daily oral doses from a quarter of a milligram up to 54 milligrams. The peptide leaves the bloodstream fast — about a three-minute half-life after an IV injection in an animal model — because the body chews it up from one end. None of that translates into a recommendation. Read every figure below as "studied at X in [species] by [route]," not as a protocol.

## Doses studied, by species and route

Reported as research context, never as guidance:

- **Obese (ob/ob) mice, oral:** foundational preclinical dosing with synthetic hGH 177-191 in the ob/ob obesity model [8].
- **Obese mice and beta-3 AR knockout mice, intraperitoneal:** 14-day chronic IP administration [4].
- **Humans, intravenous (Phase I):** single doses across roughly 25-400 mcg/kg in early Metabolic Pharmaceuticals studies.
- **Humans, oral (Phase II):** daily oral doses ranging from 0.25 mg up to 54 mg across studies; a 24-week trial used 0.25 mg, 0.5 mg, and 1 mg/day [5].
- **Rabbit knee OA, intra-articular:** 0.25 mg per knee, weekly for 4-7 weeks, with or without 6 mg hyaluronic acid [14].

The human oral program is the centre of gravity here — and it is the program that ended without an efficacy win [9].

## Half-life and routes studied

Half-life is short. Approximately 3 minutes after intravenous injection in a pig pharmacokinetic model; the intact peptide is rapidly cleared, degrading by sequential removal of amino acids from the N-terminus in a cascade fashion [7]. The disulfide bridge gives reasonable stability in lyophilised form, but in plasma the peptide is rapidly degraded from the N-terminus by aminopeptidases.

Routes studied: oral (the primary route in the human obesity program), intravenous (Phase I human PK and pig PK), intra-articular injection (the rabbit osteoarthritis model), and intraperitoneal or continuous infusion (preclinical mouse studies). Subcutaneous human pharmacokinetics have not been published in the peer-reviewed literature — a gap worth noting given how often community use assumes a subcutaneous route.

## What the dosage record does not contain

It does not contain a validated human dose for any indication. It does not contain long-term human dosing data beyond about 24 weeks. It does not contain published subcutaneous human pharmacokinetics. And it does not contain evidence that any dose produces meaningful human fat loss — the pivotal trial that tested the obesity hypothesis at defined oral doses did not beat placebo [10]. Anyone reading dose figures here should read them as the historical record of what was studied, not as a template for use.

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A brushed-steel worksheet on the AOD-9604 record — the antilipogenic fragment logged first, the receptor-sparing design stated as the design it is, and the failed human weight-loss trial left in plain steel; no clinic behind the console and nothing here dosed, compounded, prescribed, or sold.